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Simple ligation has a high failure rate, however, and modern procedures usually rely on stitching the tubes closed or severing a section of the tubes, making the possibility of reopening the pathway remote.
Such procedures usually rely on heuristic decisional criteria, hence demanding for independent checks with validation purposes.
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Advocates of a methodology of discovery usually rely on a distinction between different justification procedures, justification involved in the process of generating new knowledge and justification involved in testing it.
Studies on the activation of human B cells by innate immune receptor ligands usually rely on the use of primary B cells, which need to be purified in complicated procedures and are only available in limited numbers and in purities well below 100%.
However, the suggested methods usually rely on centralized models [4].
Applications of runoff models usually rely on long and continuous runoff time series for model calibration.
Consequently, paleontologists must usually rely on stratigraphy to date fossils.
These usually rely on expert opinion.
Protein sequence alignments usually rely on a substitution matrix.
Furthermore, researchers usually rely on care co-ordinators to access potential study participants.
Health institutions usually rely on the latter approach in an attempt to retain the workers.
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