Finally, we verified our Taqman genotyping procedure using direct DNA sequencing.
Based on the goat study, a procedure using direct injection of adult stem cells into the patient's knee to repair meniscus and prevent OA progression is currently in a phase I/II clinical trial.
The procedure uses direct current, so it has positive and negative electrodes and can have both inhibitory and excitatory effects: in general, positive current stimulates neural activity while negative current inhibits it.
This work provides systematic design procedures by using direct Lyapunov's method with a non quadratic Lyapunov's function.
Oriented Langmuir-Blodgett films [2], mechanically aligned films, e.g., stretched films [3] or films using direct rubbing procedures [4], substrate induced ordered films [5], and epitaxial vapor deposited films, [6] are widely known.
Prevalence of use by state of residence was adjusted by maternal race or ethnicity using direct adjustment procedures (Szklo and Nieto, 2007) and the racial or ethnic distribution of all NBDPS comparison mothers combined as the standard population.
Five diagnostic procedures were used: direct smear examination, the Knott's modified test, filtration assay, and two heartworm antigen detection kits.
Different vegetal materials like peanuts [12], herbage [13], yeast [14], Artemia shrimp [15] were successfully investigated for fatty acids contents by direct transmethylation in one-step procedure, using as direct transmethyltion reagents: BF3/MeOH, 5% HCl-MeOH/Toluene, 2%H2SO4/MeOHH and HCl-MeOH/Benzene.
Freshly isolated mouse splenocytes were analyzed by direct labeling procedures using primary antibodies anti-mouse CD3 (Invitrogen, Karlsruhe, Germany), anti-mouse CD120α (BioLegend, San Diego, CA, USA), and anti-mouse CD95 (BD Pharmingen, Heidelberg, Germany) and their respective isotype controls.
Outpatients were referred by their own family physician for a procedure using a direct-access or open-access referral without prior evaluation in our hospitals.
