Sentence examples similar to procedures the protein from inspiring English sources

For ELISA procedure, the protein concentration of homogenates was calculated by the Bradford method.

For quantification only the procedures separating the protein species before digestion have the advantage that the quantity of the protein species can be determined.

Western analysis was performed using standard procedures and the protein of interest was normalized to β-actin protein level.

SDS/gel electrophoresis and Western blotting were performed according to standard procedures with the protein amounts indicated.

Standard immunohistochemical staining procedures for the protein analysis were applied and utilized the following antibodies: the primary antibody was anti-human UCK2 antibody (anti-hUCK2 rabbit IgG affinity purify No. 2, Taiho Pharmaceutical Co., Ltd., Tokyo, Japan).

After 1 hour of incubation at 56°C the collection device was centrifuged and the resulting filtrate solution used to complete the extraction process, taking up the procedure at the protein precipitation step and completing as described previously.

By these procedures the total protein yield of the small vesicular fraction was about 1.2  μg per  1 · 10  parasites.

This procedure reads the protein sequence within a window of a given number of residues and computes the parameters for the amino acids within that window, then slides forward one residue and repeats the process.

The alignment procedure finds the protein reading frames encoded by the sequences that make up the isolate.

The eventual impact of the modification procedure on the protein integrity should be thus taken into account during structural interpretation.

The detection procedure of the protein bands was performed using an enhanced chemiluminescence system, and banded proteins were developed on X-Ray film using a Xomat machine (Amersham, Pharmacia, Biotech).