Sentence examples for procedures see supplementary from inspiring English sources
Comparison of barcode read counts revealed no significant difference between the two experimental procedures (see Supplementary Table S1).
We prepared AcS CH2 4Fc(CH2)4SAc from native ferrocene in three steps using well-established procedures (see Supplementary Methods, Supplementary Fig. 8 and refs 47 and 48).
In the same animals, mRNA detection followed FISH procedures (see Supplementary Methods) and used c-Fos (ACD, Catalog number: 403591), VGLUT1 (ACD, Catalog number: 317001), or GAD2 (ACD, Catalog number: 435801) probes.
For human tissue preparation and staining procedures, see Supplementary materials and methods (Additional file 1).
For DNA construction, behavioural assay and calcium-imaging procedures, see supplementary information online and references.
For detailed electroporation protocol, rat tissue preparation and staining procedures see Supplementary materials and methods (Additional file 1).
We inferred ten time-calibrated phylogenies for the 5,284 taxa dataset using a two-stage Bayesian approach and all taxonomically informative, non-conflicting fossil calibration points available in the Agaricomycetes (eight fossils, determined by a fossil cross-validation procedure, see Supplementary Fig. 4, Supplementary Table 2 and Supplementary Note 4).
The expression of these 14 genes was measured by RT-Q-PCR in tumours 4, 7, 21, 22 and 30 and in a reference set of 5 non-amplified gliomas (for an example of the procedure, see Supplementary data S2).
When the purified APC proteins are separated by SDS-PAGE and the entire gel lane is processed by in-gel digestion procedure (see supplementary Figure S3), we frequently identify a background of a small number of the same proteins.
The user has an option to include mappability information into the normalization procedure (See Supplementary Methods).
tACS-intensity was adjusted below individual phosphene- and discomfort threshold using a staircase procedure (see Supplementary Information) and ranged from 1.35 to 2 mA (peak-to-peak).
