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Three pre-fractionation procedures, SDS-PAGE at the protein level, and strong cation exchange chromatography (7 runs) [ 28] and isoelectric focusing (7 runs) [ 29] at the peptide level, were employed prior to nanoLC-MS/MS analysis to extend the proteome coverage.
I do not understand the procedure on page 7, with clustering followed by statistical tests (ch-square or wilcoxon).
The SK was characterized by sodium dodecyl sulfate polyacrylamide gel electrophoresis procedure (SDS-PAGE) on 4 12% acrylamide gradient gel under reducing conditions [ 20, 21].
Native-10% PAGE was performed according to standard SDS-PAGE procedures, but the gels did not contain SDS.
Because of iterative procedure, some memory pages have been updated/modified, called dirty pages are regenerated on the source server during migration iterations.
Therefore, the network (MME, mobile controller) first has to perform the paging procedures to inform the new traffic toward the UE and then when UE initiates the UE‐triggered service request procedure upon reception of paging indication as described in [1] and [19].
Details and procedures for SDS-PAGE and immunoblotting can be found in the Extended Experimental Procedures.
In nicotinic acid hydroxylase, Se is lost during protein storage and during simple SDS-PAGE procedures [ 20].
The procedures for SDS-PAGE, CoomassieBlue (Pierce, Rockford, IL, USA) staining, heme staining, Western blot analysis and measurements of heme content and protein concentration were described previously (Shi et al., 2006; Liu et al., 2012).
