In addition, the procedure of purification of 3-NT modified CKm resulted in the identification of an apparent trimeric form of CKm, suggesting that 3-NT modifycation may lead to the oligomerization and aggregation of this enzyme.
Hence, the possibility remains that the procedure of purification of CD45RA+ T cells could have resulted in contamination of the culture with memory CD4+ T cells, thus affecting the results [ 67].
Thus, coupling an intelligent biological-activity guided screening with an early efficient dereplication approach avoid spending time in labour intensive procedure of purification and structural elucidation of already known metabolites.
The successful implementation of in situ PPI analysis using the combinational method of site-specific tfmF incorporation and 19F NMR demonstrated that this method could be a valuable general method for conformation and function studies of soluble multi-domain proteins or protein complexes in bacterial crude lysate, without procedures of protein purification.
Tabtoxin is a relatively unstable molecule in vivo and during the procedures of toxin purification [ 40, 41].
The proteins from the RKIP-3xFLAG group, 3xFLAG group, and blank group were purified according to the FLAG M2 magnetic beads manual procedures of protein purification (each experimental step was conducted at 2 8°C), respectively.
Together with the development of procedures for purification and concentration, we have demonstrated efficient gene delivery of the luc reporter gene, constructed as described here, to the liver in mice (Fig. 1C in ref. [58]).
Procedures for purification of alkaline stable GPLs, and alditol acetate analyses of sugar moieties by gas chromatography/mass spectrometry (GC/MS) were performed as described by Eckstein et al [ 8].
The procedures for purification of CspE are discussed.
The procedures for purification of BsaS protein were identical to the procedures described for the YscN protein with one exception: the bacterial culture was scaled up to 10 L to compensate for the low expression levels of the recombinant MBP-BsaS protein fusion.
