Sentence examples for procedures of protein from inspiring English sources
The successful implementation of in situ PPI analysis using the combinational method of site-specific tfmF incorporation and 19F NMR demonstrated that this method could be a valuable general method for conformation and function studies of soluble multi-domain proteins or protein complexes in bacterial crude lysate, without procedures of protein purification.
In addition, based on the occurrence of 3D pattern similarities and the possible existence of similarities between more than two structural motifs, the results of Geomfinder help to unveil more subtle connections between proteins, and therefore be useful for novel procedures of protein classification.
The purification procedures of protein samples are illustrated in File S1.
The procedures of protein induction and cell lysis to obtain a cell extract were basically the same as the purification of the MORF library proteins.
In analogy to procedures of protein NMR, uniform labeling with N would yield AX spin systems at the imino sites of guanosine (G N H) and uridine (U N H).
The proteins from the RKIP-3xFLAG group, 3xFLAG group, and blank group were purified according to the FLAG M2 magnetic beads manual procedures of protein purification (each experimental step was conducted at 2 8°C), respectively.
Puig, O. et al. The tandem affinity purification (TAP) method: a general procedure of protein complex purification.
Thermally triggered reversible phase transition of elastin-like polypeptide (ELP) allows for a simple, economical and scalable procedure of protein purification.
This step may add some complicacy in the procedure of protein detection with iLAMP-nanoprobe method.
The procedure of protein in-gel digestion often causes the loss of post-translationally modified peptides [35], [36].
We observed the cyan fluorescence label of membrane protein ECFP-SQS surrounding the red-labeled protein bodies indicating the preservation, either partial or complete, of the membrane envelope during the isolation procedure of protein bodies.
