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The procedures of isolation and culture of HFFs were shown in the Supplementary File.
The detailed procedures of isolation and purification of the cells are provided elsewhere.
All procedures of isolation, labeling, hybridization, staining and scanning were performed by the microarray analysis core facility of the Transfusion Service of Upper Austria, Linz.
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Thus, the origin and procedure of isolation of thyroid stem cells still remain obscure.
The hearts were then excised and subjected to the procedure of isolation of ventricular myocytes by enzymatic dispersion as previously described [67].
Although Oct-4 was previously suggested to be a limiting factor during the procedure of isolation of rat ES cells [14], it appears that in addition to its function as pluripotency factor, it might have a role for the self-renewal of rat XEN cells.
The procedure of isolation of naive and memory T cells was described previously (Araki et al., 2009).
The standard procedure of isolation of the peritoneal mononuclear cells (MNC) by centrifugation in density gradient of Ficoll-Urografin d-1,078 d-1,078erformed.
In the 2011 German Shiga toxin-producing E. coli (STEC) O104:H4 outbreak, the pathogen was identified by the standard epidemiology procedure of isolation, culturing, and genotyping (Rubino et al., 2011).
The 50 mg of P14 polymer which particle size ranged from 90 to 250 µm and which was placed in a 1 mL glass column showed the best values of recovery in MISPE procedure of isolation and preconcentration of the above-mentioned analytes from the SBF samples.
Our group used BrdU-labeling and Hoechst staining for integrity assessment of mechanical isolated preantral follicles to control culture impacts [ 21], but it was shown that the procedure of isolation from the ovarian cortex itself can cause a loss of integrity in primordial follicles [ 22].
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