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The detailed procedures of genotyping were referred to in the GWAS study [ 6].
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The general analytical procedure consists of genotyping, quality control, imputation, association test and meta-analysis.
Overall, the paucity and quality of association data in ALI/ARDS call for more and better designed studies with larger sample sizes with unambiguous identification of the studied variants and procedures that allow monitoring of genotyping quality for a consistent replication and with better statistical analyses.
These STRs have also proved to be helpful in classification of Musa germplasm to distinguish individual accessions and in the development of a standardized procedure for genotyping.
However the studies should be carefully designed from the very beginning in all the steps of the procedure: pre-genotyping, genotyping and the mathematical analysis of the results.
Procedures of SNP genotyping and determination of SNP alleles using the Infinium II assay were previously described (Song et al. 2013, 2015).
The procedures of DNA extraction, genotyping, allele calling, and the linkage map have been described previously [ 30].
Genotyping quality control procedures included genotyping 10% duplicates for accuracy checking and inclusion of both positive and non-template controls in each 96-well plate.
We developed a procedure for reliably genotyping individuals of Eurasian lynx (Lynx lynx) from samples obtained through a hair-trapping scheme based on a network of lynx scent-marking sites.
The procedure of DNA extraction, genotyping, allele calling and map positions has been outlined previously [ 26].
Deviations from Hardy Weinberg Equilibrium (HWE) in the control group were assessed for quality control of genotyping procedures.
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