The one-step procedure for extraction/methylation of wet tissues was compared with the traditional procedure of extraction of the lipids by the Folch method (chloroform/methanol, 2 1, v/v), followed by methylation.
Not to mention the intrusive procedure of extraction.
After the automatic procedure of extraction and manual check, 28 proteins satisfying our requirements (see M&M) were tested with six web tools (JAFA, GOTCHA, PhyDBAC, GOblet, PFP, InterProScan) and two local tools (ARGOT and Blast2GO).
The procedure of extraction was conducted for two consecutive years (2009 and 2010).
The procedure of extraction of total RNA using the RNA extraction reagent ISOGEN Nipponn Gene, Tokyo, Japan) wasdescribed in our previous study (Fukuoka et al., 2001).
The procedure of extraction and assay of NAD, NADH, NADP and NAPH was performed according to the method described by Gibon and Larher (1997).
Chloroform fraction and hexane fraction were enriched with phenolic compounds of red propolis during liquid-liquid extraction and was dependent of the partition coefficient (K) of the phenolic compounds in the solvents chosen for this procedure of extraction.
The procedure of extraction of soil samples (10 g) with 100 mL of 0.1%K2SO44 for 24 h followed by the colorimetric assay of nitrate and ammonium concentrations in the filtrates was used.
For technical and logistic reasons, it was not possible to do twice the procedure of extraction, purification, and amplification on a consequential number of individuals to estimate precisely genotyping error rates.
