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The original ABTS method was thus improved, and in particular the procedures of cell disruption and storage were optimized.
Induced sputum (IS) using the cytospin technique has been extensively employed to characterize inflammatory airway diseases; however, procedures of cell enrichment based on cytospin increase the analytical costs and require slide processing within a short period of time after sampling.
Detailed procedures of cell culture and androgen treatment are described in the recent PLoS publication [25].
After homogenization, the lysate was incubated at 65°C for 20 min. The procedures of cell lysis, RNase A treatment, and protein precipitation followed the manufacturer's protocol for Drosophila melanogaster with necessary modifications according to the beetle weight range [6].
The procedures of cell culturing were conducted according to previous reports [ 25, 26].
Differences in the procedures of cell collection could contribute to this finding.
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Characterization of intracellular ice formed during the cooling procedures of cells significantly benefits the development and optimization design of cryopreservation or cryosurgery techniques.
The procedure of cell culture and treatment of nanoparticles was similar with the above phase of MTT assay.
In their work, a cell zooming server which is a virtual entity in the network controls the procedure of cell zooming.
Procedure of cell treatments with chemicals at indicated concentrations was illustrated in Fig. 1A, including Vitamin C (VC, Sigma, A4403), -epigallocatechin gallate (EGCG, Sigma, E4143), Vitamin E VEE, Sigma, T1782), N-Actyl-L-cysteine (NAC, Sigma, A7250), Metformin (Met, Tocris, 2864), Rapamycin (Rap, Tocris, 1292), and Resveratrol (Res, Sigma, R5010).
The pretreatment procedure of cell cycle analysis was following the manual of Cycle TestplusDNA Reagent Kit (Becton Dickinson).
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