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Collection and testing procedures of blood and plasma that are designed to exclude donations contaminated by viruses provide a solid foundation for the safety of all blood products.
The procedures of blood sample collection were reported elsewhere [43], [44].
The non-stressed control mice were treated by same experimental procedures of blood collecting and corticosterone determination with the stressed mice.
All procedures of blood and tissue sampling were performed on rats that were deeply anesthetised using vaporized isoflurane.
In summary, the preanalytical procedures of blood sampling and processing may have apparent and strong effects on the results of metabolomics studies.
Drawbacks of autologous products include potential larger individual variability in the quality of PRP compared to allogeneic products prepared from healthy blood following standardized working procedures of blood establishments [ 21].
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To avoid any distortion of the information on a patient', it is important to ensure that the experimental procedure of blood sampling at a health center, storage and/or transport, cell sorting, and RNA extraction does not affect the activation state of the immune cells in a sample.
In this study, we used a standardized retro-orbital procedure of blood collection, and calculated reference intervals for numerous biochemical and hematological parameters in 90 animals of the mouse strains C57BL/6J, 129SV/EV and C3H/HeJ in three periods of a 1-year life span.
This prompted us to analyze the common effect of anesthesia and procedure of blood withdrawal (venous versus arterial).
Hypercholesterolemia could have been analyzed but that would have involved the invasive procedure of blood sampling and the associated ethical restrictions.
To simplify the procedure of blood vessel replacement operation and shorten the vascular anastomosis time, we developed a special artificial blood vessel which can be connected to native blood vessels without suture.
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