Isolation of genomic DNA is a multi-step procedure including cell disruption, DNA extraction and DNA recovery.
Tag-seq gene expression values were strongly correlated between these two lines (Pearson r = 0.94), demonstrating that the experimental procedure, including cell line establishment, library construction and sequencing, is highly reproducible.
Procedures for western blot analysis, including cell harvesting, have been described [ 38, 42].
The procedures used for immunoblot analyses, including cell collection and lysis, collection of proteins, fractionation by SDS-polyacrylamide gel electrophoresis, and transfer to nitrocellulose membranes, have been described previously [ 39].
The experimental protocol for the slide method of the alkaline comet assay requires that all procedures during and after irradiation, including cell lysis, are carried out at 0°C; therefore, it has been widely assumed that repair of DNA single-strand breaks is impossible.
Complete blood counts were determined by standard procedures and included: cell counts (white cells, erythrocytes, leukocytes, platelets, neutrophils, lymphocytes, monocytes, eosinophils and basophils), hemoglobin, hematocrit and erythrocyte indices (mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration).
Nuclear medicine procedures including white blood cell imaging have been successfully used for the identification of bone infections.
31 Other investigators have reported potentially serious adverse events with mobilization procedures including leukocytosis, thrombocytopenia, tumor cell mobilization, splenic enlargement, and very rarely, splenic rupture.
In vitro anticancer drug chemosensitivity assays mainly involve the basic procedures including (1) isolation of cancer cells from a tumor sample, (2) incubation of cancer cells with anticancer drugs, (3) evaluation of cancer cell viability, and (4) interpretation of the results [ 1].
Subsequent procedures included mammalian somatic cell nuclear transfer, cell fusion, induction of pluripotency by ectopic gene expression, and direct reprogramming.
