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These approaches require invasive procedures for tissue biopsies, which are not always readily accessible for sampling, could be of insufficient quality, and require time for mutation analysis.
Although TMAs have many advantages over conventional manual IHC methods, direct application to clinical diagnosis has been restricted and TMA has remained a research tool because of practical limitations, such as the requirement of fast diagnostic results for urgent patients, sample damage, and tedious procedures for tissue collection.
There is no SOP (Standardized Operation Procedures) for tissue collection.
The procedures for tissue microdissection, DNA extraction, aCGH and the data analysis were as noted above.
Cells were embedded in paraffin according to standard laboratory procedures for tissue fixation.
Procedures for tissue collection, sectioning and ISH followed those described in Yaylaoglu et al. [ 19].
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The experimental data were analysed using a non-linear regression procedure for temperature effects and a general linear model procedure for tissue zone difference or the effects of storage treatments.
Although the procedure for tissue section preparation is tedious and lengthy, once prepared, the probe labeling, hybridization and signal detection steps can be easily scaled to handle multiple genes for comparative analysis.
The procedure for tissue harvesting was a previously described [ 11].
The standard procedure for tissue collection was fixation in 4% phosphate-buffered formalin.
The trichrome technique of Wheatley for fecal specimens is a modification of Gomori's original staining procedure for tissue.
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