The overall procedure for probe staining took about 2 hours, and the total volume of waste is less than 10 ml; while the conventional Masson's Trichrome and Picrosirius Red stain on OCT sections took about the same time, but required few more steps of staining and washing.
The horseradish peroxidase amplification was replaced with quantitative PCR, procedures for removing excess probe were examined, and new probes were synthesized.
This method, already used in previous works, allows a simple procedure for atom probe sample preparation [20].
The same procedure was used for probe preparation for the VG-V subfamily of VicTR-B repeats (using clone c609 [GenBank: DQ139394]) and for VicTR-A clones c653 [GenBank: EU568805] and c666 [GenBank: EU568818].
Hybridization intensities were generated for the experiments according to the standard procedures for GeneChip expression probe arrays.
The detailed procedures for ATP affinity probe labeling, tryptic digestion, and affinity purification of desthiobiotin-labeled peptides were reported previously.
The detailed procedures for RNA purification, probe preparation and data acquisition using the Affymetrix E. coli genome 2.0 array are described in Supplementary Methods.
Quantification of the Fl emission ratios of 1 and 2 allows us to deduce the GSH concentration, following previously reported procedures for Ca2+ ratiometric probes.
The procedure, illustrated in Figure 2 for probe PSMB4a, is as follows: residual errors are obtained by subtracting 1000 from each data point in database BB.
As described in their product literature, the Affymetrix probe set design procedures for the three-prime arrays attempt to generate probe sets for all known or inferred expressed sequences.
