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Routine procedures for observation by electron microscopy were performed by Filgen Inc (Nagoya, Japan).
Frozen-hydrated samples of SNARE-containing vesicles prior to, and approximately 35 s after, Ca2+ addition were prepared using the procedures for observation in cryo-EM as described previously (Kyoung et al., 2011).
Frozen-hydrated samples of synaptobrevin-2 v-vesicles in the presence of 20 μM α-Syn were prepared using the procedures for observation in cryo-EM as described previously (Kyoung et al., 2011).
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Observation and Experience: The experimental procedures for the observation and experience conditions of this study have been described earlier [17], but are briefly illustrated in Figures S2 and S3.
European Parliament's election observation activities are governed by the Decision of the Conference of Presidents of 13 September 2012 which sets out clear criteria and procedures for election observation delegations.
The two general components for collecting such data are the design structure for the study (possible population manipulation, and where and when observations are to be made) and the procedures for making observations (population assessments) in each location at each time.
Details of sensitivity and the procedure followed for observations are given in Pawar et al. (2010).
As discussed, the size of the heart in children is usually small and the access routes for the procedure are limited for observation.
After confirming the specificity of these polyclonal antibodies to their target proteins by western blot analysis (Figure 1E), trophocytes were isolated from worker bees eight days after eclosion, fixed, and stained with these antibodies according to a standard procedure [3] for observation by confocal microscopy (CM) (Leica TCS SP2 MP).
Suggested procedures for conducting classroom observations, materials for faculty on guiding the work of GSIs, and resources for developing a 300-level course on teaching can be found on the GSI Teaching and Resource Center website.
While formal records of monitoring associated with silvicultural work and budgets have always been maintained, a record keeping and summarization procedure for incidental observations was not established until the fall of 2010.
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