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For example, laboratory procedures for mutation scanning tests often involve a primary screen to determine which fragments carry mutations, followed by a second confirmatory test by sequencing to characterize the mutations present.
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The procedure for mutation was conducted according to the manufacturer's instructions (MutanBEST, TaKaRa Biotechnology) and the mutants were sequenced for verification.
Although the allele specific qPCR is an efficient closed-tube procedure for mutation identification, this fluorescent based method still costs false positive results [ 9].
The procedures for iterative mutation of S. mobaraensis genome with ARTP mutation breeding system purchased from Si Qing Yuan Biotechnology Co., Ltd.
When a number of different estimation procedures for passenger mutation rates were used, it was found that approximately 120 genes were more frequently mutated than the passenger rate, suggesting that these genes are more likely to carry driver mutations (so-called CAN genes).
In accordance with previous studies (Krypuy et al, 2006; Do et al, 2008; Ma et al, 2009), we confirm that HRMA is a reliable, sensitive and rapid procedure for KRAS mutation detection.
We derive theoretically optimal procedures for combining rare mutations and construct suitable test statistics for various biological scenarios.
Compromising the potential use of iPSCs for cell replacement therapy are the suboptimal procedures for iPSC production, mutations during reprogramming and uncertainties over the genomic stability of the differentiated derivatives.
The aim of this study was the exploration of the screening procedure for CF mutations employed in our laboratory as a first-line diagnostic instrument for Cystic Fibrosis.
The procedure of mutation is summarized as follows: Firstly, a chromosome is randomly extracted.
To develop an accurate mutation analysis procedure for retinoblastoma gene (RB1) mutation, which is sensitive at the single-cell level, and to use in vitro fertilization (IVF) and preimplantation genetic diagnosis (PGD) to achieve pregnancies without retinoblastoma.
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