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Standard procedures for manipulation of nucleic acids were essentially those of Sambrook et al. [ 31].
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The experimental instrumentation, including the loading device, and the procedures for the manipulation of experimental data are detailedly described; experimental results obtained on a steel specimen and a composite laminate are also reported.
Procedures for the manipulation of ES cells were based on published protocols [34].
Procedures for DNA manipulation were carried out as described previously [13].
All procedures for animal manipulation and experiments are in accordance with the COBEA, the Brazilian institution that regulates animal experimentation.
Most standard operating procedures for animal manipulation require that at least 2 persons be present, regardless of their experience level.
All of the procedures for the manipulation of animals were consistent with the Ethical Principles in Animal Research adopted by the Brazilian College for Animal Experimentation COBEAandasd asuggesteded by the APA Guidelines for Ethical Conduct in the Care and Use of Animals.
Specimens were obtained from the Museo de Historia Natural "Javier Prado", Universidad Nacional Mayor de San Marcos (UNMSM) in Lima, Peru and the Field Museum of Natural History in Chicago (FMNH), US, and collected by L.L. in northern Peru following the American Society of Mammalogists' guidelines [ 84] and University approved procedures for the manipulation of mammals (see Appendix).
The cryptography community has long developed rigorous privacy models and provably secure procedures for data manipulations.
The procedures for DNA manipulations were performed essentially as described by Sambrook et al. [ 56].
Plasmid DNA isolation and general procedures for DNA manipulations were essentially performed as described previously [ 16].
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