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The procedures for genotyping in the SHFS have been described previously [ 46].
Quality assurance procedures for genotyping PON1 SNPs included assessment of randomly distributed blank samples and duplicates with independently isolated DNA from the same subjects.
Measures of digestive efficiency in individual cages, blood sampling procedures for genotyping and euthanasia procedures by injection of pentobarbital were approved by the ethics committee in Animal Experimentation of Val de Loire (00886.02 and 01047.02).
Quality assurance procedures for genotyping both PON1 SNPs included assessment of randomly distributed blank samples in each plate and duplicates of randomly selected samples with independently isolated DNA from the same subjects.
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These STRs have also proved to be helpful in classification of Musa germplasm to distinguish individual accessions and in the development of a standardized procedure for genotyping.
Our procedure for genotyping these mice has been described previously [14], [20].
We have since used this procedure for genotyping and mapping in several other species.
Minimization of the conditional variance of the genotypes in target animals provided an effective optimization procedure for prioritizing animals for genotyping or sequencing.
A modified high-salt DNA extraction procedure [ 33] was adopted for genotyping individual insects.
We thus conclude that the optimized PCR MP procedure may offer a discriminatory method for genotyping of yeasts in epidemiological studies, as well as in the control of nosocomial infections.
We developed a procedure for reliably genotyping individuals of Eurasian lynx (Lynx lynx) from samples obtained through a hair-trapping scheme based on a network of lynx scent-marking sites.
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