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Procedures for flow cytometry were adapted as previously described[ 24].
(ii) Improvement and simplification of the standardization and calibration procedures for flow cytometry.
To confirm that the anti-CD20-conjugated antibodies were binding specifically to surface-expressed CD20 antigen, isolated PMBCs were washed and pre-incubated with 5 μg/mL RTX Hoffman-La Rochee, Basel, Switzerland) on ice for 15 minutes and washed again before beginning the staining procedures for flow cytometry analysis (Accuri flow cytometer; BD Biosciences, San Jose, CA, USA).
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Two greener procedures for flow-injection spectrophotometric determination of nitrite in natural waters were developed and critically compared.
A detailed dual drainage simulation model is described based upon hydraulic flow routing procedures for surface flow and pipe flow.
This paper presents an innovative numerical procedure for flow simulation and estimation of relative permeability of naturally fractured carbonate rocks by history matching laboratory derived multi-phase production data.
The procedure for flow cytometric analysis was performed as described previously [16].
The procedure for flow cytometry (FCM) staining has been described [32].
The procedure for flow cytometry (FCM) staining followed the method described by Liu [ 14].
(A ) Sequential steps of gating procedure for flow cytometry analysis and sort of mammary epithelial cells stained with anti-CD31, anti-CD45, anti-CD24 and anti-ICAM-1 antibodies.
The procedure for flowing the washing solution, the volume of which was limited on the CD-type microchip, was optimized by using a recycled spinning-pausing rotation program to overcome the non-specific adsorption of the horseradish peroxidase labeled APnEOs at the detection area.
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