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Standard procedures for cloning and analysis of DNA, PCR, electroporation and transformation were used.
Detailed procedures for cloning are described in the Supporting Information.
General procedures for cloning and DNA manipulation were performed as described previously (18).
A detailed diagram [see Additional file 1; Figure 5], summarizes these procedures for cloning and analysis of enriched ChIP DNA.
General procedures for cloning, DNA isolation, amplification and manipulations, transformations, agarose and protein gel electrophoresis were performed as described by Sambrook et al. [ 32].
Procedures for cloning and purification of plasmids were followed as reported in manuals [ 19]. pCMV-Luc was a gift from Dr. Robert Debs.
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The detailed procedures for cloned, large insert genomic DNA isolation entailed a modified cleared lysate procedure as described in detail earlier [ 34, 35].
Procedure for cloning Gloeophyllum trabeum actin-encoding genomic DNA.
Procedure for cloning Gloeophyllum trabeum glyceraldehyde-3-phosphate dehydrogenase-encoding genomic DNA.
The detailed procedure for cloning and propagation has been described previously (Ogawa et al, 2002; Shi et al, 2003).
MAF clone management models the laboratory procedures for clone handling through wells, plates and plate sets.
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