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There are two basic procedures for cell formation in group technology.
Using 24 normal adult guinea pigs we assessed three surgical procedures for cell delivery into the cochlea: (i) a cochleostomy into the scala tympani (ST); (ii) direct access to Rosenthal's canal – the site of the SGN soma – via a localized fracture of the osseous spiral lamina (RC); and (iii) direct access to the auditory nerve via a translabyrinthine surgical approach (TL).
The origin of BHK-21 cells and procedures for cell growth in Dulbecco's modification of Eagle's medium (DMEM), and for plaque assays in semisolid agar have been previously described [81], [92].
The procedures for cell isolation for A375 tumour xenografts were previously described (Hida et al, 2004).
The procedures for cell counting were the same as for explants.
Reagents and procedures for cell culture were described previously (Ge et al., 2013).
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Cells were cultured in monolayer in either chondrogenic or basal medium, recapitulating procedures used in existing clinical procedures for cell-based therapies.
Designing effective cryopreservation procedures for cells requires knowledge of permeability of cell membrane to water and solutes.
To design effective cryopreservation procedures for cells and tissues, it is critical to know permeability of cell membrane to water and solutes.
Although a standardized procedure for cell counting was used, comparably high variations were observed.
The procedure for cell isolation and culture was performed as described previously [ 21].
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