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Figure 1 is a trial procedures flow chart.
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The overall procedure flow of our unified framework contains superpixel pre-segmentation, color contrast and color distribution computation, combination, and final refinement.
Following this procedure, flow cells were placed in the sequencer and fluorescently-labeled bases were attached to the complementary bases of each sequence.
The rest of the procedure flows directly into the established chemistry, microarray probing with biotinylated cRNAs, and analytic algorithms developed for transcriptional profiling.
Procedures for flow cytometry were adapted as previously described[ 24].
(ii) Improvement and simplification of the standardization and calibration procedures for flow cytometry.
Every attempt was made not to have intervention sessions interfere with clinic procedures and flow.
Surface staining was performed according to standard procedures and flow cytometric analysis was done on a Coulter Cytomics FC500.
For understanding the experimental procedures, a flow diagram was provided in the Additional file 1: Figure S1.
Interruptions also have a negative impact on work procedures, work flow, ability to concentrate, reflective processes, and interaction with patients (28, 37).
Rigorous standardization procedures in flow cytometry and gene expression microarrays are applied to build this resource, resulting in a comprehensive database of the gene expression profiles in the murine immune system.
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