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Flares caused by free agents were not observed in either of the confirmation procedures (Figure 2D).
Comparing the three procedures (Figure 8) the HCOOH method appeared to be the less effective in extracting Acid Orange 7. Figure 8 Comparison between the extraction methods.
To clearly compare the CSEP methodology and the new testing procedures, Figure 2 shows the PDF of occurrences and log-likelihoods computed by the CSEP LBT and the proposed procedure for the first ETAS simulated Japanese catalog.
In fact there was severe degradation of the Precision in the analysis of the data normalized by all these procedures (Figure 4D).
The labeling of cells with both vital dyes did not block cell proliferation and the dyes were brightly observed after the fixation procedures (Figure S2).
As such, it seems reasonable to postulate that standard mitochondrial isolation procedures, which by definition must disrupt mitochondrial structure due to the mechanical nature of the homogenization and centrifugation procedures (Figure 1), could have quite dramatic effects on mitochondrial function.
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Supplemental Experimental Procedures, Figures S1 S5 Table S1.
Experimental procedures, Figures S1 S11, and Table S1.
Supplemental Experimental Procedures, Figures S1 S4, and Tables S1 S4 Document S2.
Supplemental Experimental Procedures, Figures S1 S4, and Tables S1 and S2.
Supplemental Experimental Procedures, Figures S1 S4, and Table S1 Movie S1.
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