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Tat-P-TEFb and AFF42-73 were purified separately following procedures described recently (Schulze-Gahmen et al., 2013).
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The simulated systems were equilibrated for 5 ns following a procedure described recently.
The detailed procedure was described recently.
The first was doped with heteroatoms boron and sulfur (B/S) following the procedure we described recently, while the second was doped with heteroatoms boron and selenium (B/Se).
Gels were electrophoresed at 30 V for 30 min and then at 50 mA per gel for 45 min, and stained using Vorum silver staining procedure as described recently [ 29].
Though quite a few arthroscopic procedures have been described recently using suspensory fixation devices, an extremely high level of accuracy is required in terms of placement of tunnels.
For plasmid PFGE an S1 nuclease treatment procedure was used as described recently [ 54].
The procedures were similar to those described recently [11], [53].
When a highly efficient gene transfer was required (Western blot), plasmids were introduced to freshly isolated neurons (DIV0) using the AMAXA nucleofection procedure (Lonza, Basel, Switzerland) as described recently [ 25].
The preparation of recombinant proteins of SARS-CoV and the procedures for Western blot assay have been described recently (14 ).
as we have described recently [29].
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