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DNA values were calculated by comparison to the human lymphocyte as a standard (3.50 pg DNA per nucleus) which were prepared by the same procedures but using the biological saline pH 7.4 as PBS instead.
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While the classroom-based program and the online program share a similar curriculum, the online program does not focus on North Carolina laws or procedures, but uses examples from a variety of different states.
Further PPI analyses repeated this procedure, but using time courses from the left AIFO, the preSMA and the right pLOFC ROI coordinates as seeds.
Finally, IVP blastocysts were vitrified using conventional fibreplugs to maintain a high embryo recovery rate, and then warmed using the one-step warming in-straw CP dilution procedure, but using an adapter with a wider opening coupled to the French straw and a heated metal chamber to protect and keep the straw at 41 °C (experiment 4).
The synthesis of this reagent followed the known procedure, but using 1.2 equivalents NaH instead of NaOH and 1.1 equivalents BnOH in THF, respectively.
A media fill is the performance of an aseptic procedure mimicking the conditions of the real procedure, but using a sterile microbiological growth medium instead of the solutions otherwise used in the preparation of the radiopharmaceutical.
Bone marrow derived imDCs were also analyzed with the same procedure but using CPMV-AF488.
Sham microinjections followed the same procedure but using 2 µL of sterile saline 0.9%.
Control samples were subjected to the same procedure but using a non-specific IgG pull-down.
Ligands were biotinylated using the same procedure but using a twofold molar excess of sulfo-NHS-LC-biotin.
Size disparity was calculated using the same procedure, but using average Condylobasal Length as the single variable.
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