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Lung sections were de-paraffinised with antigen retrieval by standard procedures as previously described [21].
using standard neurosurgical procedures, as previously described [51].
Kinetic constants Km, Vmax, and Ki were calculated using procedures as previously described [27].
Identification of fungi present in BAL or blood samples was performed using conventional culture procedures as previously described [23], [24].
Proteins were isolated from myotubes using a protein lysis buffer and Western blot analysis was carried out using standard procedures as previously described [16].
PCR products were blotted onto nylon membranes and hybridised with 19 digoxigenin-labelled sequence-specific oligonucleotide probes, using hybridisation and chemiluminescence procedures, as previously described.
The resulting DNA fragments of the psbA gene were used for chlamydomonas chloroplast transformation without further purification steps and cloning procedures, as previously reported [47].
The posterior distributions of the different parameters of interest were estimated via MCMC procedures as previously described [13] from 2,000 post burn-in samples (with a burn-in period of 2,500 iterations) and a thinning interval of k = 25.
The parabiosis was performed following procedures as previously described [ 31].
cDNA was synthesized using standard procedures as previously described [ 24].
DNA was extracted by standard procedures as previously described [ 28].
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