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Following incubation, graft segments were subjected to three washing/gamma counting procedures and scanning electron microscopy (SEM).
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Gels were stained using Coomassie Blue or silver stain according to standard procedures and scanned using an ImageScanner (Amersham Biosciences).
A "core laboratory" was set up for quality control procedures and scan interpretation in order to obtain uniformity and high-quality results across the CTN.
All sample labelling, hybridization, staining and scanning procedures were carried out using Affymetrix standard protocols (www.affymetrix.com).
Biotin-labelling, hybridization, washing, staining and scanning procedures were performed according to the Affymetrix technical manual.
Sample labelling, hybridization, staining and scanning procedures were carried out using Affymetrix standard protocols.
Through literature review and local experience, a standard operating procedure and scanning service has been developed.
The substrate from the microcosm was sampled after cessation of the experiment (53 weeks) from the bottom and top layers of selected columns and was analyzed using sequential extraction procedure and scanning electron microscopy-energy-dispersive X-ray spectroscopy method.
Procedures for hybridisation, washing, staining and scanning of chips were carried out according to standard protocols (Affymetrix, Santa Clara, CA).
Procedures for animal handling, anesthesia, and scanning complied with the guidelines of the European Community EUVDD 86/609/EEC) for the care and use of laboratory animals and were approved by the local authorities.
To verify the extent of applicability of the proposed procedure, both broadside and scanned array configurations are considered.
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