Sentence examples for procedures and protein from inspiring English sources

Cell lysis, SDS-PAGE and blotting, were done by standard procedures and protein bands were detected with a chemiluminescent substrate kit (Pierce) according to the manufacturer's recommendations.

Rat liver mitochondria were prepared by standard procedures and protein concentration was determined by the Bradford method [ 15].

Extended Experimental Procedures Cloning and Protein Purification Full-length rat amphiphysin 2-6 (Wiggetet al., 1997 ) and full-length rat endophilin A1 were cloned into pGEX-6P2.

Extended Experimental Procedures Cloning and Protein Preparation C. elegans SAS-6 Proteins DNA encoding full-length or fragments of C. elegans SAS-6 (Uniprot ID O62479) were cloned in pET system vectors (Novagen) encoding for N-terminal His6-tags or pGEX system vectors (GE healthcare) encoding for N-terminal GST-tags.

Western analysis was performed using standard procedures and the protein of interest was normalized to β-actin protein level.

SDS-PAGE and transfer to nitrocellulose were performed using standard procedures and equal protein loading of the wells was confirmed by Coomassie Brilliant Blue R250 staining of the gels.

Intramuscular fat (IMF %) of the muscle samples were measured using chemical fat extraction procedures and crude protein was measured on extracted samples (1 to 1.5 g) using the Macro-Kjeldahl method [ 27].

Chapters 5 - 7 discuss grass and legume forage species suitable for tropical regions, pasture management procedures, and energy-protein supplementation programs needed during the extensive dry periods.

The SPP procedures, IP tests, and protein samples for SDS-PAGE are detailed in the Text S1 Supplemental Experimental Procedures.

Procedures for mutagenesis and protein purification can be found in the Supplemental Materials.

Inflation parameter 1.5 was used for the clustering procedure and the proteins were organized into 13,752 protein families.