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Briefly, bone marrow biopsies were obtained from volunteers after obtaining written informed consents under IRB of the National Institute of Dental and Craniofacial Institute approved procedures, and processed as described previously [12].
Fasting blood samples were collected according to standardized CARDIA procedures and processed at central laboratories (15).
Fasting blood samples were collected according to standardized CARDIA procedures and processed at central laboratories (9).
DNA was extracted from buffy coats or from buccal cell samples (collected by mouthwash swish and spit procedures) and processed via the QIAmp™ (QIAGEN Inc., Chatsworth, CA, USA) 96-spin blood kit protocol.
22 23 Blood was collected in EDTA-coated tubes from HC, patients with SSc or patients with RA during standard outpatient procedures and processed within 4 h of collection.
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