Sentence examples for procedures and primer from inspiring English sources

The complete sequencing procedures and primer sequences are described at www.hivfrenchresistance.org.org

The procedures and primer sequences for LAM-PCR for LV integration site retrieval have been previously described (66).

The procedures and primers sequences have been described in full detail elsewhere [10].

PCR procedures and primers are described in Additional file 2. Sequences were edited using BioEdit [ 27].

Multi Locus sequence Typing (MLST) was performed using previously described procedures and primers [ 52].

The general PCR procedures and primers (WQ1 and WQ2) are shown in Additional file 1 (Table S1 and Table S2), respectively.

The entire 18S rRNA gene (1,796 bp) was obtained from 2 DNA fragments amplified by PCR from DNA extracted from 3 fecal specimens by using procedures and primers for genetic analysis of coccidian parasites (4, 7, 8 ).

This method was inspired by a PCR-based site-directed mutagenesis method developed by Stratagene (La Jolla, CA, USA); the procedure and primer design were modified to enable the method to generate deletions several hundreds of nucleotides in length with an efficiency of 80 100%, and to delete two DNA fragments simultaneously in a single PCR.

RT-PCR procedure and primer sequences are in SI Materials and Methods.

Procedure and primer sequence was previously described [ 19].

The PCR procedure and primers for exons 5 9 were as described elsewhere (Hsu et al, 1991).