Tumor type was scored by histopathology and by expression levels of vimentin and E-cadherin: both histopathology procedures and expression array procedures were previously described in [ 10].
Primers for the quantitative RT-PCR analysis can be found in the Supplemental Experimental Procedures, and expression was normalized to β-actin, Actb, and TATA box binding protein, Tbp.
This allowed assessment of whether the peak phase of different tissues from WT and Vipr2−/− mice was associated with a particular CT phase or was reset by cull/culture procedure and expression peaked the same number of hours after cull regardless of the CT phase of cull.
The SAFE (Significance Analysis of Function and Expression) procedure was used to identify molecular pathway perturbations.
Different prescriptions for the reconstruction procedure and expressions for numerical fluxes are tested.
A calculus is defined in terms of stipulations [ PR §202), (PG 369)], known rules of operation, and known decision procedures, and an expression is only a mathematical proposition in a given calculus (PR §155), and only if that calculus contains (PG 379) a known (and applicable) decision procedure, for "you cannot have a logical plan of search for a sense you don't know" (PR §148).
From the previous optimization procedure and the expression of each optimal beamforming vector, we can interpret the principle behind the optimal beamforming design for each scenario.
The datasets were tested for differential variability using our F-test-based procedure, and differential expression by Welch's two-sample t-test (Welch, 1947).
Cox regression analysis of factors, including age, tumor classification, tumor stage, lymphovascular space invasion, depth of invasion, adjuvant therapy, surgical procedure and CD10 expression, which were potentially related to survival, was used to identify independent factors that might jointly have a significant effect on survival.
Extended Experimental Procedures Cloning Expression and Purification of LMO2 LDB1LID, SCLbHLH, and E47bHLH LMO2:LDB1LID construct was cloned, expressed and purified as described (El Omari et al., 2010 ).
Recently, Kwon et al. (Kwon et al. 2010) established the cell-free expression of computationally predicted putative ω-TAs, which circumvents cloning and expression procedures.
