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The His-tagged D-amino acid oxidase was purified in a single step by nickel-chelate chromatography to a specific activity of 115 U/mg protein at 25°C and with a final procedure yield of 82%.
Thus, our data demonstrate for the first time that a straightforward shRNA-library construction protocol in line with a stringent cellular selection procedure yield potent and readily convertible shRNAs against a viral genome.
This discrepancy might be attributable to differences in DNA isolation procedure (yield and quality) and qPCR preparation (PCR chemistry, sample DNA load, etc).
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The process harks back to old-fashioned daguerreotype sittings and the rather formal procedure yields a stark, striking, yet informal product.
The above procedure yielded 829 articles.
This procedure yields an ensemble forecast.
This collagenase-trypsin procedure yields 3 6×106cells/heart.
This procedure yields chelation efficiencies consistently greater than 98%.
This procedure yielded 791 pairs of station sites.
This procedure yields 77 decompositions for the 1996-2004 comparison.
In a case like this, which procedure yields higher discriminability?
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