Injection solutions of the BPA-fructose complex (BPA-fr) were prepared at a concentration of 10 mg BPA/0.43 mL using a previously published procedure with modifications [6].
From all specimens, DNA was extracted from the cortical plate (fetus) or gray matter (children, adults) using a standard procedure, with modifications [51] and analyzed by MethyLight PCR after bisulfite conversion [22], [23], [52] (for primer sequences, see Table S2).
Total proteins were extracted from apoplast fluid using a phenol-based procedure with modifications [ 117].
3,6,2′,3′,4′,6′-Hexa- O-acetyl-2-deoxy-2-fluoro-α d-maltosyl fluoride was prepared according to a published procedure with modifications (Scheme S1 of the Supporting Information).
Based on the proportion of patients for whom the initial surgical plan was modified at actual surgery, CE-CT was the least suitable imaging procedure, with modifications of the surgical plan in 47 per cent of patients undergoing surgery after CE-CT as a single imaging modality.
It seeks to improve the quality and outcome of patient care through structured review of medical radiological practices, procedures and results, whereby these are examined against agreed standards for good medical radiological procedures, with modification of practices, where appropriate, and the application of new standards if necessary.
The DAB-Mn2+ procedure along with modification of this DAB method has been reviewed (Babbs 1994).
Penetration assays were performed using published procedures with modifications [ 28].
Post-attachment virus neutralization assays were carried out using published procedures with modifications [ 29, 30].
