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At this point various modifications to the procedure were preformed to enrich for virus-like particles (VLPs) by different means: Purification by filtration (FD) – Two samples were filtered through a 0.45 μm Millex®-HV low protein binding PVDF syringe filter (EMD Millipore) which was washed with 500 μl of SM-plus buffer.
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This two step amplification procedure was preformed in order to avoid non-specific amplification of the CYP2D6 pseudogene located near the CYP2D6 gene.
Standard recombinant DNA procedures were preformed as described by Sambrook et al [59].
Alpha level was set at.05 and Tukey HSD post hoc procedures were preformed on the significant main effects and interactions, when appropriate.
All procedures were preformed on a trauma simulator.
Animal procedures were preformed in accordance with institutional and UK Home Office guidelines.
All procedures involving animals were preformed in accordance with protocols approved by the University of Illinois, Urbana-Champaign Institutional Animal Care and Use Committee.
Verification of equipment and methods related performance 1 1 1 No procedures of verification for equipment and methods were preformed in all producers.
All experiments were preformed in accordance with the guidelines of the Canadian Council of Animal Care and all animal procedures were approved by the McGill University/Douglas Hospital Animal Care Committee (protocol number: 5084).
They were preformed for each other".
FAST scans were preformed in 242 cases.
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