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20 21 One study was excluded because it used a laparoscopic Duhamel-type anastomosis 22 and one study because infants undergoing a staged procedure were pooled with primary procedure results.
Second, the sequences rejected by the procedure were pooled with the sequences of the nine species already present in the Génolevures database which are not members of any protein family and a clustering with OrthoMCL [ 60] was applied to define new families.
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The results for each of these three procedures were pooled for exploratory data analysis.
After fixative procedure, embryos were pooled 10-122 embryos/perl) per genotype and then minced using forceps in a 1 1 methanol:acetic acid solution.
Cells from six electroporation procedures per LCL were pooled to obtain 1.2 × 107 cells.
The pellet was used to conduct a second extraction procedure, and the sulfated polysaccharides were pooled.
The two tanks per temperature and feeding were pooled, according to the procedure described in Underwood80, to check that variation among experimental units was zero, and that pooling was appropriate and did not change the conclusion of the analysis.
The organic fractions were pooled and the same procedures were performed to yield the respective fractions.
The multiple imputed datasets were then analyzed using standard procedures for complete data and the results were pooled.
This procedure was repeated three times, and the lavages were pooled.
Each time 2 EMBs were pooled to minimize sampling error effects as a routine procedure in our laboratory.
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