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All the procedure were done in duplicate for each single animal.
Electrophoresis and the staining procedure were done with the PhastSystem (Pharmacia) according to manufacturer's recommendations.
All steps in this staining procedure were done at a maximum volume of 200 µL.
The examinations as part of the pretransplantation procedure were done at various referring hospitals or at the transplantation center as part of their routine diagnostic work.
Microarray gene expression preparation and processing procedure were done as recommended by the manufacturer (Affymetrix, Inc .. Raw expression values were extracted and normalised by the Affymetrix Power Tools based on Robust Multiarray Average (RMA) approach.
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The procedure was done by an aesthetician.
"My procedure was done by body modification master, Steve Haworth.
Sometimes the procedure was done in homes, cars and campgrounds.
He was worried about metastasis, and recommended that the procedure be done immediately, but she balked.
The procedure was done with a laparoscope, which shortens recovery time considerably.
The procedure was done at the request of Mr. Tools's wife, Carol, Dr. Gray said.
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