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Field trials of the new procedure were conducted by researchers from nations participating in the IEA Solar Heating and Cooling (SHC Programme Taskk 22, using a number of detailed hourly simulation programs from Europe and the US, including: CA-SIS, CLIM2000, ENERGYPLUS, PROMETHEUS, TRNSYS-TUD, and two versions of DOE-2.1E.
Construction of the chamber, membrane and experimental procedure were conducted using the recommendations [16].
All cell culture procedures after this substrate sterilization procedure were conducted in a biohazard hood, using aseptic techniques.
Experiments for the optimization of extraction procedure were conducted in triplicate; briefly, the grinded dry material was suspended in KOH solutions; afterwards the suspension was sieved (0.5 mm screen) and centrifuged at 10,000 rpm for 40 min at 4°C.
All steps of the immunostaining procedure were conducted at room temperature.
All analyses other than the ARIMA procedure were conducted with SAS (version 9.1; SAS Institute, Inc., Cary, NC).
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The sequencing procedure was conducted as previously described5,52.
The stereotactic procedure was conducted following a previously published procedure20.
During an interview C2 told the investigators that a procedure was conducted in her parents' bedroom.
The procedure is conducted online based on a limited exploration of the state space.
For depth profiling experiment, a careful ion etching procedure was conducted using low ion energy beam of 500 eV.
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