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Given the simplicity of the apparatus and procedure, we conclude that the new task may be employed to assay learning and memory in adult zebrafish in an efficient manner.
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As we typically prepare 50 ml of the coupled lysate from 10 liters of L. tarentolae culture in a one day procedure, we concluded that the system was sufficiently scalable and inexpensive to be useful in multiplexed analysis [4].
Since the performance on these test sets did not differ significantly from that estimated during the optimization procedure, we concluded that we had not over-fitted the networks to the data.
Considering the technical aspects of the two procedures, we conclude that the benefits of ICWs are generalizable irrespective of the protein target of interest.
Instead, after an intensive review of our procedures, we concluded that the most likely source of this contamination was a technical lapse during collection of the urine pools.
We have determined the degree of demethylation in our analytical procedure and we conclude that the method results in about 5% demethylation of MeHg, half of it in the solubilisation step if samples are heated during solubilisation (as for hair), and the other half in the analytical step.
Comparing the performance of our procedure with this work, we conclude that the procedure used in our study is efficient and can serve as a useful tool for other systems, as well.
We conclude that the procedure only fails if S does not have full rank.
Therefore, we conclude that our normalization procedure did not impair the proportion of full-length clones compared to non-normalized libraries.
We conclude that our clustering procedure resolves even challenging gene families correctly and is a successful strategy to identify putative new gene family members in P. falciparum and other Plasmodium species.
While the biological significance of these findings awaits testing in other cellular systems, we conclude that the SPILL procedure is an efficient tool for the SIMS-based investigation of specific proteins.
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