In the sample preparation procedure, we added 2 mmol/L ethylenediaminetetraacetic acid (EDTA) to avoid the activation effect of ATPγS by chelating divalent ions (Proks et al., 2010).
In the second procedure, we added the antioxidant tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl), known to increase the lifespan of mice [ 26, 27], to cell cultures nearing senescence (experimental details are described in Additional file 1).
To account for VLP loss during purification procedures we added 10-fold excess PFU of each phage for each of the 10 individual samples in the master mix.
In addition to factors common to both procedure models, we added selected covariates to specific procedure volume analyses.
An arbitrarily large number is set as an initial value of u(t a ), and an estimate of u(t a ) is excluded from the evaluation of τ 2. The second procedure we have added to the method of Fujii and Kanda (2008) is used to remove outliers such as spikes in the data.
Using a similar procedure, we have added the +C·G base pair to LNA doublets and created seven unique triplets (+A +X+C/T YG, +C +X+C/G YG, +G +X+A/C YT, +G +X+C/C YG, +G +X+G/C YC, +G +X+T/C YA, and +T +X+C/A YG).
To remove the red blood cells from the neutrophils, we used a water lysis procedure, in which we added 20 ml of distilled water to the neutrophil and red blood cell pellet and mixed gently for 20 s, followed by the quick addition of 20 ml of Hank's balanced salt solution (HBSS) 2× solution while continuing mixing, for a final concentration of 1× HBSS (pH 7.4).
For example, Bell (1987, page 155) rightly would find "disturbing … the impossibility of 'messages' faster than light, which follows from ordinary relativistic quantum mechanics in so far as it is unambiguous and adequate for procedures we [emphasis added] can actually perform.
In a second series, we added a procedure in which subjects assigned descriptive labels to the stimuli.
Indeed, due to the specific context we are dealing with, i.e. the gene prioritization, we could not use the classical cross-validation procedure, we in fact added 15% of the disease genes randomly drawn for 10000 times from the disease gene set to the candidates, and counted each time the number of the diseases that fell in the top four windows of 100, 75, 50 and 8 genes.
To acquire more accurate result, we add interpolation procedure in Mapping.
