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MudPIT: cells were lysed in RapiGest™ SF cell lysis buffer as per the manufacturer's procedure (Waters Corporation, Milford, MA).
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Before the docking procedure, water molecules were removed and zinc ions were retained.
The latter procedure (water extracts) was the least cumbersome, hence it was selected for further experiments.
During the dialysis procedure, water was exchanged at 3-h intervals for 2 days and then the resulting solution was lyophilized for 3 days.
Therefore, after the degassing procedure, water will have a better chance of penetrating deeper than 5 to 10 nm into the vacancy left by the detached nanobubbles.
According to the manufacturer's procedure, water extraction of Stemona tuberosa was performed.
In the BKM-react reconciliation procedure, water and protons are removed from the reaction equation prior to comparison, and reactions with the same compounds are grouped, irrespective of the precise stoichiometry of the compounds.
This indicates that in the initial steps of the drying procedure water is first being removed from the cores of lumens and, at the end of the process, the only water molecules remaining in the samples are those located inside the cell walls under different degrees of mobility, with the most confined water populations non-covalently bound to cellulose fibers.
Calorie Test: In this procedure water is introduced to the ear canal and the movement of the eyes are noted.
A detailed description of the methodology, including selection and enrollment procedures, water, and biological sample collection and processing, has been previously published (Wasserman et al. 2011).
This can be at the regional level or across regions on operational or sectoral issues such as financing procedures, water and sanitation, education, etc. and could be included as a part of the 5% of staff time that they are supposed to allocate to training and learning with the support of their Representatives.
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