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At each step of the procedure, validation with experimental data is carried out, confirming the accuracy of the approach.
The accuracy of the proposed MLR model is illustrated using various evaluation techniques: leave-one-out cross validation procedure, validation through an external test set and Y-randomization.
Procedure validation was performed using the Std CRM 145 R reference material containing added known amounts of metal [ 76].
A centralized, blinded PCR respiratory quality control panel is also provided to all sites to ensure procedure validation on site before specimens are processed locally.
After an initial procedure validation phase in parallel with the National TB Reference Laboratory (NTRL) and legal approvals, the laboratory registered for external quality assessment (EQA) from the NTRL, WHO, National Health Laboratories Services (NHLS), and the College of American Pathologists CAPP).
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The models refinement procedure and validation were performed by cross-validation.
Analysis procedure and validation with the aCGH has been described previously (Lips et al, 2011a).
The details of our conditions for the microarray procedure and validation studies were previously described [ 6- 8, 21, 22].
AO and AB developed the procedure, performed validation studies and were involved in all the experiments presented here.
Details of the quality performance of instrumentation and their calibration are equally important for the procedure's validation.
These tasks cover operations and maintenance procedures; overall validation; and installation and commissioning.
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