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The immunofluorescence procedure utilized the Histogene LCM Immunofluorescence Staining Kit (Molecular Devices).
The procedure utilized the acetonitrile precipitation of plasma proteins and trypsin digestion protocol by Kay et al. [ 16] and was described in detail previously [ 9, 11].
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Percutaneous renal core biopsy will be performed directly before the IRE procedure, utilizing the procedural anaesthesia.
The unfolding procedure utilizes the pull-move set as a neighbourhood relation and a new population-based search method.
The equivalent thermal conductivity is determined in an iterative procedure, utilizing the results of the laboratory experiment as input data of the computational model.
For improved transient response featuring low peak response and fast attenuation, the design procedure utilizes the mode equalization followed by the maximization of the damping.
The numerical procedure utilizes the wave function expansion of the exciting as well as scattered fields, and the boundary conditions at the periphery of holes are incorporated as the relations between the expansion coefficients of exciting and scattered fields.
The procedure utilizes the results of a standard closed-contouring approach, but then adjusts the elevation of the volcanic base by evaluating the shape of the edifice along a series of topographic profiles.
The proposed energy-based homogenization procedure utilizes the sensitivity filter technique, especially, a modified optimal algorithm is proposed to evolve the microstructure of lattice materials with distinct topological boundaries.
In turn, the effective properties of the resulting three-scale LCC are determined by means of a sequential homogenization procedure, utilizing the self-consistent estimates for the effective behavior of the polycrystalline matrix, and the Willis estimates for the effective behavior of the porous composite.
The procedure utilizes the points for which most of the non-dominated solutions produce same class labels to train the SVM classifier with a particular kernel.
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