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Eco-efficiency analysis was used as an optimisation tool to create more sustainable solutions and improve subsequent reactions, enabling the scaled-up biocatalytic process to become more economical and competitive compared with the chemocatalysed procedure using sodium iodate.
CeO2 nanoparticles, denoted further as "Ce-NC" and "Ce-NR" for nanocubes and nanorods, respectively, were prepared via hydrothermal procedure, using sodium hydroxide as the precipitating agent, as we described elsewhere [30].
Virus DNA was extracted from cell-free supernatant virus preparations following a standard phenol/chloroform procedure using sodium dodecyl sulfate (SDS) and proteinase K lysis, phenol/ether extraction, and ethanol precipitation [ 23].
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RNA was precipitated by standard procedures using sodium acetate and ethanol for precipitation.
The procedures for protein analysis using sodium dodecyl sulfate−polyacrylamide gel electrophoresis (SDS−PAGE) on 15% gels (19) and protein quantification are reported elsewhere (20).
A standard antigen retrieval procedure using tri-sodium citrate in a microwave for 8 min was used for the human and mouse BPIFB1 antibodies as well as the MUC5AC, CD68 and CCSP antibodies.
This procedure, involving clearing of fixed tissue using sodium hypochlorite followed by mPS-PI staining and mounting in high refractive index mounting medium provides a rapid means to image wall ingrowth deposition in TCs without the need to use more time-consuming electron microscopy techniques.
However, when the simultaneous loading-reduction procedure was used, sodium borohydride influenced the acidity remarkably by formation of a new strong acid site, resulting in better dehydration performances.
All surgical procedures were performed under general anesthesia using sodium pentobarbital (50 mg/kg i.p .. Normothermic partial hepatic ischemia was induced by performing a midline laparotomy, exposing the liver hilum and subsequent clamping of portal structures to the left and median lobes with a microvascular clip, yielding ~70% hepatic ischemia [ 27].
HRP-labeled LD5 (HRP-LD5) was prepared by using sodium periodate according to the following procedure.
Soy proteins are extracted from soybean oil bodies using sodium carbonate buffer, similar to the procedure described by Herman [ 11].
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