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Protein concentration was determined by using the Bio-Rad Protein Assay, based on the Bradford dye-binding procedure using bovine serum albumin as standard.
The protein concentration was determined by the Lowry procedure using bovine serum albumin as the standard [ 39].
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Protein concentration in thymus homogenates was measured following the standard procedure [ 50] using bovine serum albumin as the reference standard.
The soluble and immobilized protein concentration was determined by the dye-binding procedure of Bradford using bovine serum albumin as a standard, as described previously [ 10].
Protein concentrations were determined according to procedure described by Bradford using bovine serum albumin as the standard [26].
After incubation for 30 min on ice, the extracts were centrifuged at 13.200 r.p.m. for 20 min. Protein concentration of supernatant was measured by the Bradford procedure Bio-Rad Laboratoriess, Richmond, CA, USA), using bovine serum albumin as a standard.
Protein concentration was determined according to the procedure of Bradford (12) using bovine serum albumin as the standard.
All procedures were initially optimized using bovine lens lipid extracts.
Protein concentration was determined according to the procedure described by Bradford [25] using bovine serum albumin as the standard.
Specific enzyme activity was expressed as U mg−1 protein, based on the determination of protein concentration according to the procedure described by Bradford [ 32] using bovine serum albumin as the standard.
This method [ 25] was used to analyze the interactions between the ligand and HSA. 5) Protein analysis Protein concentration was determined according to the procedure described by Bradford [ 26] using bovine serum albumin as a standard.
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