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The Ago2 IP procedure used herein should, however, be widely applicable to other cells or even whole organisms, in which mRNAs identified as targets, by either overexpressing or blocking a specific miRNA, can be related to specific biological consequences.
Although prior assessments of microsecond motions have been limited by poor NMR sensitivity and nonexponential decays (for T1ρ(C)) or averaging of values by spin diffusion (for ⟨ T1ρ(H ⟩), the Lee Goldburg (LG) spin-lock procedure used herein preserves site-specific information while allowing practical measurement of T1ρ(H s through the Cs directly bound to the respective H nuclei.
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Two scores were used herein following standard procedures for scoring the ILC [ 39]: (1) The ILC 0-100 scorepresentingting a total QoL score, is obtained by summing the rating values across the seven items and transforming them into a 0-100 scale (0 = Very low QoL, 100 = Very high QoL).
37 The models used herein were constructed from the crystal structure of Ls-AChBP (PDB-ID 1I9B) following a procedure similar to that reported in Ref. 40 and building on our own previous work.
The criterion used herein is that used in [28].
The norm used herein is that used in reference [17].
Table 3 shows the mesh details used herein.
All symbols used herein are summarized in Table 1.
This section presents the research design and methodology used herein.
Other names used herein may be trademarks of their respective owners.
Moreover, the parameters used herein are the basic clinical variables.
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