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The procedure under "construction of calibration graphs" for the first approach was then conducted.
The spectra of these mixtures were then recorded and the procedure under construction of calibration curves was then followed but using the recorded spectra of the prepared mixtures.
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The above procedure described under "Construction of the Calibration Graphs" was then performed.
The procedure mentioned under "construction of calibration graphs" for the first approach was then applied.
Different aliquots of the working standard solution were analyzed using the procedure mentioned under "Construction of calibration graph".
Applying the procedure mentioned under construction of a calibration graph, the relationship between NF concentration and fluorescence intensity was found to be linear.
The remaining eight laboratory prepared mixtures, Table 1, were chosen to be used as an external validation set and the procedure given under construction of calibration set was then followed.
The procedures described under "Construction of calibration graphs" were followed adopting any of the three methods.
The subsequent dilutions were performed via diluting an appropriate volume of this solution with ethanol and the procedures described under "construction of calibration graphs" were then applied.
The procedure described for method I under Construction of the Calibration Graph was performed.
Further dilution with the mobile phase was done to obtain the working standard solution to be analyzed as described under the section "General procedure and construction of calibration graphs".
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