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One procedure unchanged since Edward Lloyd's day is the Names' unlimited liability.
Leaving all other steps of the calibration procedure unchanged, the addition of HS-DNA during DNA extraction yielded significant improvements of the calibration curves.
As false positive discovery is always a concern for high throughput analysis, we performed a control experiment here with the sample preparation procedure unchanged except without adding PNGase F to the sample in order to estimate the extent of non-specific deamidation.
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The HCl concentration was also tuned from 0 to 1.35 M, keeping all other experimental parameters and procedures unchanged.
As control, adipocytes slides were not incubated with the primary antibodies, but with IgG isotype 1 and the rest of procedure remained unchanged.
Otherwise, the likelihood function in Eq. (1) and the maximization procedure remain unchanged.
Although the basic procedure appears unchanged, probabilistic assessment requires more precise definitions of each step and thus also promotes greater transparency as to the biological and quantitative assumptions underlying the dose response assessment.
Proteolytic release of GLA enzyme from the ZERA fusion could then be achieved at the TEV cleavage site using a monomeric TEV protease; the specific activity after this polishing procedure remained unchanged.
Secondary antibody controls were performed by replacing the primary antibody with TPBS/0.1%BSA (the carrier buffer for the primary antibodies), while all other procedures remained unchanged.
Rates for the other procedures remained unchanged.
As standard REC procedures were unchanged, investigators were not informed about this study.
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CEO of Professional Science Editing for Scientists @ prosciediting.com