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To facilitate a more comprehensive analysis of the underlying mechanism we designed a screening procedure to enrich for phenotypes with defects in induced egress.
We developed a procedure to enrich the COG database with UniRef50 clusters from the UniProt database [ 9], creating the UECOG database [ 10].
In this study, we evaluated the potential involvement of monocytic EPCs in SSc vasculopathy by examining their quantity as well as their angiogenic and vasculogenic properties using the procedure to enrich circulating precursors for monocyte-derived multipotential cells.
The viral DNA was isolated using a previously described procedure to enrich for viral DNA that is packaged in nucleocapsids [ 18, 19]; this method generally produces sequencing libraries that contain 10% or less contaminating sequence reads from the host genome.
Comprehensive assessment of the extent of lateral gene transfer can be made easily by genomic subtraction, a procedure to enrich sequences that are present in one genome but not in another by using biotinylated subtractor DNA to fish out the target DNA by hybrid formation.
Moreover, our group has been developing a procedure to enrich secondary databases such as COG [ 12] and KEGG Orthology (to be published) with UniRef50 clusters [ 16] available from UniProt, therefore allowing the inclusion of data from incompletely sequenced genomes.
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The authors thank Yizhong Zhang for extracting organelle-enriched DNA, Derek Schmidt for early work to assess extraction procedures to enrich for organellar DNA, Amy Hilske and Samantha Link for procuring and caring for plants in the Beadle Center Greenhouse, and members of the Mower lab and the Sally Mackenzie lab for helpful discussions.
Furthermore, LSVM is enhanced with data-mining and bootstrapping procedures to enrich models during the training.
Furthermore, the identification of mechanical properties distinct to stem cells could result in more successful sorting procedures to enrich multipotent progenitor cell populations.
Recently, procedures to enrich definitive endoderm for effective differentiation of ESCs into ATIICs have been developed by using a growth factor cocktail or a lung-specific cell-conditioned medium[43], but are not yet successful in generating a homogenous population of ATIICs.
There are several procedures to enrich for the plasmid containing the library insert.
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