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Whole genome amplification (WGA) is an in vitro procedure to amplify a genomic DNA (gDNA) sample to generate amplified DNA (wgaDNA) for further molecular genetic analyses, and has been considered by some as a potential solution to the problem of limiting gDNA availability.
The labeling was performed using Eberwine procedure to amplify the starting material.
We modified the well-established neurosphere procedure to amplify the PRPs of the postnatal SVZ.
An enrichment step was performed on samples that were negative by DAL procedure to amplify the number of coliphage in the samples.
We used this procedure to amplify the genomes of 37 single cells from sections of frozen mouse tumor and normal lung tissues, which were micro-dissected and catapulted onto micro-tube caps.
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Molecular procedures to amplify, clone and sequence these genes are described elsewhere [41].
The PCR procedure was able to amplify the 0.7-kb B. cinerea fragment form mixed samples of DNA as low as 2 pg B. cinerea genomic DNA and 1 μg plant DNA.
This procedure allowed us to amplify the strength of the searched signal.
This procedure was used to amplify a specific fragment of the NS5 gene within the flavivirus genome.
The most common strategy to circumvent the large material requirement using a standard procedure has been to amplify the starting mRNA.
Since the COMT promoter sequence was not available in databases, a walking-PCR procedure was performed to amplify the 5'-flanking region.
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